Presenting authors are underlined.
Abstract 3: 鈥淭he Influence of Incline and Load on Speed, and Energy Expenditure鈥.听 Naomi Kim and Cara Wall-Scheffler.聽Walking surfaces are often inclined, affecting speed, energy use, and gait. This study examined how incline and load interact to influence pedestrian speed and cost of transport (CoT). Twelve participants walked a 300m loop with a 20% incline and decline, both loaded and unloaded. Speed decreased with incline (p=0.004), especially with head loads (p=0.09). CoT increased with both incline and load (p<0.001), but no interaction was found (p=0.2). These results suggest incline and load independently affect cost, while their interaction primarily influences gait. Understanding these effects is crucial as humans adapt to diverse environments.
Abstract 4: "Design matters: Energetics of infant-carrying slings in bipedal locomotion".聽 Elizabeth Delgado, Sara Dean, Sydney Banks and Cara Wall-Scheffler.聽Infant-carrying slings have long supported human mobility by enabling efficient transport of offspring. This study investigates how sling design influences walking kinematics and energy expenditure in bipedal locomotion. Participants walked a 30m course carrying a 5kg infant-model under three conditions: unloaded, traditional sling, and loop-style carrier. Each sequence was repeated in reverse order to control for order effects. Results showed that load significantly increased the cost of transport (p=0.014), with no change in walking speed (p=0.8) or percentage of gait cycle spent in stance or swing phases (p>0.8). Torso tilt was significantly reduced in the loop condition (p=0.025), suggesting that sling design influences postural control and locomotor efficiency.
Abstract 5: 鈥淭he distracted stride: How phone use changes the way we walk鈥.听 Gursagar Singh, Elizabeth Delgado, Eloisa Nguyen and Cara Wall-Scheffler.聽Modern use of cellular devices has altered natural gait patterns, often restricting arm swing and leading to changes in gait stability. Measures of stability, including stride width, length, and speed, were collected from three conditions: No Phone (NP); No Arm Swing (NAS); Phone Reading (PR). A repeated measures ANOVA showed that gait speed significantly decreased during PR (p<0.001); step width trends differed by sex as females narrowed their step during PR, whereas males widened their step width during NAS (p=0.192). These findings suggest that walking with a phone perturbs the gait beyond that caused by not swinging the arms.
Abstract 6: 鈥淏eyond the Marine Norm: Salinity Tolerance in Freshwater-Adapted Sticklebacks鈥.听 Gursagar Singh, Ali Al-Tamimi, Sunny Cheema and Cara Wall-Scheffler.聽"Stickleback fish are studied to better understand the range of phenotypic plasticity. Though often found in marine environments, Blakely Island hosts sticklebacks that have recently evolved to live in freshwater. Our research investigated whether freshwater sticklebacks can manage higher salinities through physiological adjustments. Swimming activity was measured in varying salinities (0%; 17.5%; 35%) in sticklebacks of varying sizes across a 24-hour period. An ANOVA was run to determine the effects of salinity, fish size, and diurnal rhythm on activity. The interaction between these three variables significantly predicted activity (p=0.022). Larger (older) fish struggled at high salinity, while smaller (younger) fish excelled."
Abstract 7: 鈥淥ptimizing selective whole genome amplification to increase MinION sequencing coverage of the Mycoplasma genitalium genome for mutation analysis鈥.听 Alexis Nield, Isabel Williamson, Mia Ewing, Noah Soltani, Derek Wood, and Gwendolyn Wood.聽Mycoplasma genitalium is a sexually transmitted bacterium that causes urethritis, cervicitis and pelvic inflammatory disease. Selective Whole Genome Amplification (SWGA) can be used to specifically amplify the MG genome from low copy clinical samples for use in downstream sequencing. This study aimed to optimize the SWGA protocol to improve coverage of the MG genome, especially the 16s gene region known to harbor antibiotic resistance mutations. Of the variations tested the temperature ramping protocol improved amplification in the 16s region by four qPCR cycles which equates to a 10-fold increase in genome copies at this key location.
Abstract 8: 鈥淎mplification of Mycoplasma genitalium from Aptima Test Kit Samples for MinION Sequencing鈥.听 Sammi Do, Erik Garcia, Olivia Brooks, Itzel Aparicio, Derek Wood, and Gwendolyn Wood.聽We developed a method to isolate Mycoplasma genitalium (MG) DNA from Aptima test kits, which contain a 1:1 ratio of patient urine to specimen transport medium. The extracted DNA undergoes selective whole genome amplification (SWGA) to enrich MG DNA for sequencing on a MinION platform. This approach ensures sufficient DNA yield for downstream analysis. By sequencing the amplified DNA, we can identify mutations associated with antibiotic resistance, providing a valuable tool for studying MG and informing treatment strategies.
Abstract 9: 鈥淨uantifying SWGA Enrichment in Mycoplasma genitalium Using qPCR鈥.听 Madeline Vogl, John Lee, Madeline Crawford, Feng Chen, Derek Wood, and Gwendolyn Wood.聽Mycoplasma genitalium (Mg) causes persistent cervicitis, urethritis, and pelvic inflammatory disease in humans. Selective Whole Genome Amplification (SWGA) of clinical samples allows whole genome sequencing from the limited number of Mg found in urine. We developed a qPCR assay targeting rpmA that allows rapid confirmation of SWGA success. This gene shows a 1000-fold DNA increase post-SWGA as compared to standard 16s primers. These findings allow rapid evaluation of SWGA protocol improvements without the need for sequencing.
Abstract 10: "Succession and forest maturation decreases population density of black-tailed deer on Blakely Island, WA".聽 Isabella Burnside and Eric Long.聽Early successional habitats have previously been demonstrated to provide plentiful foraging opportunities for ungulates such as Colombian black-tailed deer (Odocoileus hemionus columbianus). The removal of predators paired with food abundance can substantially increase deer density. As habitat succession continues, however, the population density may decrease as the forest canopy closes and restricts food source growth. Over the last 17 years Blakely Island, WA, has undergone successional habitat loss as the forest regrows after the 2006 ban on large-scale logging. During the summer of 2024, we used line-transect distance sampling along with a pre-existing dataset to estimate the population density of black-tailed deer in response to forest maturation. Since 2010 we have seen a deer density decrease of 4.0% per year as the forest matures, which has disrupted the island鈥檚 ecosystem.